Endoproteinase AspN from New England Biolabs

Product Specs
ItemEndoproteinase AspN
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberP8104S
Molecule NameEndoproteinase AspN
Quantity50 µg
ApplicationsProteomics & Glycomics
Brand/TrademarkNew England Biolabs Inc (NEB)
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Amyloid fibril proteomics of AD brains reveals modifiers of aggregation and toxicityMolecular NeurodegenerationSeptember 14, 2023Arun Upadhyay, Deepak Chhangani, Nalini R. Rao, Julia Kofler, Robert Vassar, Diego E. Rincon-Limas, Jeffrey N. Savasα-chymotrypsin (Sigma, Cat#C3142), thermolysin (Sigma, Cat#P1512), endoproteinase Asp-N (New England Biolabs, #P8104S), Glu-C (Sigma, Cat#P2922), Arg-C (Biovendor, Cat#RBG40003005), trypsin (Promega, Cat# V5280), and Lys-C

(2) Placental Remote Control of Fetal Metabolism: Trophoblast mTOR Signaling Regulates Liver IGFBP-1 Phosphorylation and IGF-1 BioavailabilityInternational Journal of Molecular SciencesApril 14, 2023Fredrick J. Rosario, Anand Chopra, Kyle Biggar, Theresa L. Powell, Madhulika B. Gupta, Thomas Janssonwas then diluted with 2X Asp-N reaction buffer (NEB, B8104S) and digested with 10 ng of Asp-N (NEB, P8104S) overnight at 37 °C. The resultant peptides were desalted with C18-ZipTip (Millipore Sigma, ZTC18S096),

(3) Site-specific N-glycosylation analysis of animal cell culture-derived Zika virus proteinsScientific ReportsDecember 1, 2021Alexander Pralow, Alexander Nikolay, Arnaud Leon, Yvonne Genzel, Erdmann Rapp, Udo ReichlSequencing Grade Modified, V5111, Promega, Madison, WI, USA) endoproteinase AspN [ Flavastacin20] (AspN, P8104S, New England Biolabs, Ipswich, MA, USA), proteinase K (A4392, AppliChem, Darmstadt, Germany) and peptide

(4) Unexpected Receptor Functional Mimicry Elucidates Activation of Coronavirus FusionCellFebruary 1, 2019Alexandra C. Walls et al.TG269188 Chymotrypsin,Sigma-Aldrich,Cat# 9004-07-3 Alphalytic Protease,Sigma-Aldrich,Cat# A6362 AspN Endoproteinase,NEB,Cat# P8104S EndoH,NEB,Cat# P0702S Lot# 0181603 EndoF3,CalBIOCHEM,Cat# 324727 Lot# 2754801 Proteinase K,ThermoScientific,Cat#

(5) Improvement of the glycoproteomic toolbox with the discovery of a unique C-terminal cleavage specificity of flavastacin for N-glycosylated asparagineScientific ReportsDecember 1, 2017Alexander Pralow, Marcus Hoffmann, Terry Nguyen-Khuong, Erdmann Rapp, Udo Reichlwere trypsin (Trypsin Sequencing Grade Modified; V5111) from Promega and endoproteinase AspN (AspN; P8104S) from New England Biolabs (Flavastacin, purified host cell protein, Uniprot Q47899; see the corresponding

(6) Assessing in vivo dynamics of multiple quality attributes from a therapeutic IgG4 monoclonal antibody circulating in cynomolgus monkeymAbsJuly 3, 2016Yinyin Li, Yu Huang, Janine Ferrant, Yelena Lyubarskaya, Yue (Emma) Zhang, Siyang (Peter) Li, Shiaw-Lin (Billy) Wupurchased from MP Biomedicals (Solon, OH); Endoproteinase AspN was purchased from New England BioLabs Inc. (P8104S) and Roche Diagnostics (11054589001). Dithiothreitol (DTT, D5545), iodoacetamide (IAA, I6125) and N-ethylmaleimide

(7) Enzyme-Induced Formation of β-Lactoglobulin Fibrils by AspN EndoproteinaseFood BiophysicsDecember 1, 2008Cynthia Akkermans, Paul Venema, Atze Jan van der Goot, Remko M. Boom, Erik van der LindenBiophysics (2008) 3:390–394 50 mM Tris–HCl and 2 mM zinc acetate. The enzyme AspN endoproteinase (P8104S, New England Biolabs, Ipswich, MA, U.S.A.) was dissolved in Millipore water (0.17 mg/ml) and was added

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